Barium oxide (BaO) and zinc sulfide (ZnS) are well known for their applications in electrical, optical and chemical industries. The present study was aimed to evaluate the impact of biofield treatment on the structural and physical properties of BaO and ZnS powder. The study was carried out in two groups, one was set to control, and another group was subjected to Mr. Trivedi’s biofield treatment. Control and treated samples of BaO and ZnS were analyzed using X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), and surface area analyzer. XRD data showed that lattice parameter and unit cell volume of BaO powder were reduced upto 0.42% and 1.26%, respectively as compared to control. Whereas, density of treated BaO was increased upto 1.27% as compared to control. Besides, the unit cell volume was changed in treated ZnS from -0.55 to 0.24% as compared to control that led to change in density from -0.24 to 0.55% after biofield treatment. However, the crystallite size was substantially increased upto 40.5% and 71.4% in treated BaO and ZnS, respectively as compared to control. FT-IR data exhibited that absorption peaks at wavenumber 862/cm (control) was shifted to 858/cm in treated BaO. Upward shifting of absorption peaks corresponding to Zn-S stretching bond was observed in treated (617/cm) as compared to control (592/cm). Furthermore, surface area result showed that it was reduced by 4.32% and 2.1% in treated BaO and ZnS powder, respectively as compared to control. Hence, these, findings suggest that biofield treatment has altered the structural and physical properties of BaO and ZnS powders.