Herbomineral formulations have been used worldwide against various chronic and degenerative diseases, due to its fewer side effects. A new proprietary herbomineral formulation was formulated, consisting of essential ingredients viz. herbal root extract of ashwagandha and minerals (zinc, magnesium, and selenium). The present study aimed to evaluate the impact of Biofield Energy Treated herbomineral formulation in male Sprague Dawley (SD) rats for immune biomarkers modulation. The test formulation was divided into two parts. One part was denoted as the control without any Biofield Energy Treatment, while the other part was defined as the Biofield Treated sample, which received the Biofield Energy Healing Treatment remotely from twenty renowned Biofield Energy Healers. The immunomodulatory biomarkers like alteration in immune biomarkers such as IgG and IgM, CD4+ and CD8+, hematology, lipid profile, hepatic enzymes, sex hormone, and antioxidant profile. The humoral immune response of IgM and IgG were altered by 4.54% and 9.49%, respectively in the Biofield Energy Treated test formulation (G4) group compared with the disease control (G2) group. On the other hand, the cellular immune response i.e. CD4+ and CD8+ counts were significantly increased by 56.21% and 73.6%, respectively in the G4 group compared with the G2. Hematology analysis showed an increased level of TLC and lymphocytes by 14.85% and 21.42%, respectively in the G4 group compared to the G2. Lipid profile analysis showed a significant decrease in the level of total cholesterol, triglycerides, LDL, and VLDL in the G4 group compared with the G2. Further, results suggests a significant decrease in the hepatic biomarkers such as SGOT, SGPT, and cardiac enzyme, CK-MB by 18.54%, 19.12%, and 26.23%, respectively in G4 group compared to the G2 group. However, the testosterone level was significantly decreased in G4 group and untreated test formulation (G5) groups by 53.87% and 26.25%, respectively compared with the G2 group. Additionally, the antioxidant assay data showed an altered level of tested enzymes such as LPO, SOD and CAT, in the G4 group compared with the G2 group. Therefore, it can be concluded that the Biofield Energy Treated test formulation showed a significant improved cellular and humoral immunity, hematological and biochemical profile as compared with the untreated test formulation. As a result, it can be established that The Trivedi Effect®-Biofield Energy Healing has the significant capacity for immunomodulatory effect, which may also be useful in organ transplants, anti-aging, and stress management by improving overall health and quality of life.