The present study aimed to evaluate the impact of The Trivedi Effect®- Energy of Consciousness Healing Treatment based new proprietary herbomineral formulation and Energy Healing Treatment per se in male Sprague Dawley rats for immune biomarkers modulation. The test formulation was divided into two parts, one as the control without any Biofield Energy Treatment, while the other part was defined as the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment remotely from seven renowned Biofield Energy Healers. Additionally, one group of animals also received Biofield Energy Treatment per se (day -15) by under similar conditions. Humoral immune response data showed an increased level of IgG by 2.89%, 6.20%, and 6.20% in the Biofield Energy Treated test formulation group (G4), Biofield Energy Treatment group per se (G6), and Biofield Energy Treated test formulation at day -15 (G7), respectively as compared with the disease control group (G2). The ratio of CD4+/CD8+ was increased by 3.80%, 12.38%, and 16.19% in the G4, G6, and G7, respectively compared with the G2. Hematology analysis suggested an increased level of TLC and neutrophil by 9.64% and 1.48%, respectively in the G4 group, while G6 group showed an increase count of TLC, lymphocytes, and monocytes by 19.79%, 2.87%, and 15.54%, respectively compared with the G2. Biochemical study showed an increased concentration of glucose by 11.78%, 18.60%, and 56.84% in the G4, G6, and G7, respectively compared with G2. Total cholesterol was significantly decreased by 10.54% and 4.20% in the G4 and G7, respectively compared with G2. In contrast, the HDL level was increased by 11.69% and 1.06% in G6 and G7, respectively while LDL was decreased by 10.52% in G4 compared with the G2. SGOT, CK-MB, total protein, albumin, and globulin levels were decreased by 4.04%, 23.54%, 1.68%, 1.54%, and 1.80%, respectively in G4, while G6 group showed decreased level of SGOT, CK-MB, and A/G ratio by 2.08%, 16.23%, and 3.40%, respectively compared with G2. However, SGOT was significantly decreased (p≤0.01) by 18.88% in G7 compared with G2. The testosterone level was decreased by 76.67%, 18.43%, and 44.06% in G4, G6, and G7, respectively compared with G2. Antioxidant profile showed a decreased level of LPO by 59.63% in G7, while SOD and CAT levels were significantly altered in tested groups compared with G2. Biofield Energy may also be used for autoimmune and inflammatory diseases, stress management and prevention, and anti-aging by improving overall health.