Selenium (Se) is an essential trace element, and its deficiency in the humans leads to increase the risk of various diseases, such as cancer and heart diseases. The objective of this study was to investigate the influence of biofield energy treatment on the physical and thermal properties of the selenium powder. The selenium powder was divided into two parts denoted as control and treated. The Control part was remained as untreated and treated part received Mr. Trivedi’s biofield energy treatment. Both control and treated selenium samples were characterized using x-ray diffraction (XRD), differential scanning calorimetry (DSC), thermogravimetric analysis - differential thermal analysis (TGA-DTA), and Fourier transform infrared spectroscopy (FT-IR). The XRD data showed that biofield energy treatment has slightly altered the lattice parameter (0.07%), unit cell volume (0.15%), density (-0.14%), atomic weight (0.15%), and nuclear charge per unit volume (-0.21%) in the treated selenium powder as compared to the control. The crystallite size of the treated selenium powder was reduced considerably from 106.98 nm (control) to 47.55 nm. The thermal analysis study showed that the latent heat of fusion was 64.61 J/g in the control, which changed to 68.98, 52.70, 49.71 and 72.47 J/g in the treated T1, T2, T3, and T4 samples respectively. However, the melting temperature did not show any considerable change in the treated selenium samples as compared to the control. The FT-IR spectra showed the absorption peak at 526 and 461 cm-1, which corresponding to metal oxide bonding vibration in the control and treated selenium powder respectively. Hence, overall data suggest that, the biofield energy treatment considerably altered the physical and thermal properties of selenium powder. Therefore, biofield energy treatment could make selenium even more useful nutrient in human body.