Silver oxide has gained significant attention due to its antimicrobial activities. The purpose of this study was to evaluate the impact of biofield energy treatment on the physical and thermal properties of silver oxide (Ag2O). The silver oxide powder was divided into two parts, one part was kept as control and another part was received Mr. Trivedi’s biofield energy treatment. The control and treated samples were analyzed using X-ray diffraction (XRD), differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), and Fourier transform infrared (FT-IR) spectroscopy. The XRD diffractogram showed that the crystallite size of treated sample was significantly altered on the planes (200), (311), and (220) by 100, 150 and -25% respectively, with respect to control. The DSC result exhibited that the thermal energy required to decompose the silver oxide to silver and oxygen was altered from -12.47 to 71.58% in treated samples as compared to the control. TGA showed that the onset temperature of thermal degradation was reduced from 335°C (control) to 322.4°C. In addition, the rate of weight loss in treated sample was increased by 4.14% as compared to the control. Besides, the FT-IR did not show any alteration in absorption wavenumber of treated sample as compared to the control. Hence, the XRD, DSC and TGA data revealed that the biofield energy treatment has a significant impact on the physical and thermal properties of silver oxide powder. Therefore, the biofield energy treatment might improve the dissolution rate in formulation and bioavailability of treated silver oxide as compared to control.