The current research work was designed to explore the impact of The Trivedi Effect® - Energy of Consciousness Healing Treatment (Biofield Energy Healing Treatment) on magnesium gluconate for the change in the structural properties and isotopic abundance ratio (PM+1/PM and PM+2/PM) by using LC-MS and NMR spectroscopy. Magnesium gluconate was divided into two parts – one part was control, and another part was treated with The Trivedi Effect®- Biofield Energy Healing Treatment remotely by seven renowned Biofield Energy Healers and defined as The Trivedi Effect® Treated sample. The LC-MS analysis of the both control and treated samples revealed the presence of the mass of the protonated magnesium gluconate at m/z 415 at the retention time of 1.53 min with similar fragmentation pattern. The relative peak intensities of the fragment ions of the treated sample were significantly changed compared with the control sample. The proton and carbon signals for CH, CH2 and CO groups in the proton and carbon NMR spectra were found almost similar for the control and the treated samples. The isotopic abundance ratio analysis revealed that the isotopic abundance ratio of PM+1/PM (2H/1H or 13C/12C or 17O/16O or 25Mg/24Mg) in the treated sample was significantly increased by 34.33% compared with the control sample. Subsequently, the percentage change of the isotopic abundance ratio of PM+2/PM (18O/16O or 26Mg/24Mg) was significantly decreased in the treated sample by 64.08% as compared to the control sample. Briefly, 13C, 2H, 17O, and 25Mg contributions from (C12H23MgO14)+ to m/z 416; 18O and 26Mg contributions from (C12H23MgO14)+ to m/z 417 in the treated sample were significantly changed compared with the control sample. Thus, the treated magnesium gluconate could be valuable for designing better pharmaceutical and/or nutraceutical formulations through its changed physicochemical and thermal properties, which might be providing better therapeutic response against various diseases such as diabetes mellitus, allergy, aging, inflammatory diseases, immunological disorders, and other chronic infections. The Biofield Energy Treated magnesium gluconate might be supportive to design the novel potent enzyme inhibitors by using its kinetic isotope effects.