Potato Micropropagation Medium (PMM) is the growth medium used for in vitro micropropagation of potato tubers. The present study was intended to assess the effect of biofield energy treatment on the physical, thermal and spectroscopic properties of PMM. The study was attained in two groups i.e. control and treated. The control group was remained as untreated, while the treated group was received Mr. Trivedi’s biofield energy treatment. Finally, both the samples (control and treated) were evaluated using various analytical techniques such as X-ray diffractometry (XRD), differential scanning calorimetry (DSC), thermogravimetric analysis- differential thermal analysis (TGA-DTA), UV-Vis spectrometry, and Fourier transform infrared (FT-IR) spectroscopy. The XRD analysis showed the crystalline nature of both control and treated samples of PMM. The X-ray diffractogram showed the significant increase in the intensity of XRD peaks in treated sample as compared to the control. The XRD analysis revealed 6.64% increase in the average crystallite size of treated PMM with respect to the control. The DSC analysis showed about 8.66% decrease in the latent heat of fusion in treated sample with respect to the control. The TGA-DTA analysis exhibited about 4.71% increase in onset temperature of thermal degradation after biofield treatment with respect to the control, while the maximum thermal degradation temperature (Tmax) was also increased (5.06%) in treated sample with respect to the control. This increase in Tmax might be correlated with increased thermal stability of treated sample as compared to the control. The UV spectroscopic study showed the slight blue shift in λmax of treated sample with respect to the control. FT-IR spectrum of control PMM showed the peak at 3132 cm-1 (C-H stretching) that was observed at higher wavenumber i.e. at 3161 cm-1 in the treated sample. Other vibrational peaks in the treated sample were observed in the similar region as that of the control. Altogether, the XRD, DSC, TGA-DTA, UV-Vis, and FT-IR analysis suggest that Mr. Trivedi’s biofield energy treatment has the impact on physicochemical properties of PMM. This treated PMM might be more effective as a micropropagation medium as compared to the control.